Recent estimates of the number of species range from 10 to 100 million (Wilson 1998). Mitochondrial DNA is very useful for its multiple presences in cells. The cytochrome b gene on the mitochondria has been used successfully in spe- cies identification [9, 10, 11] and in taxonomic and phy- logenetic studies [12, 13, 14]. No variation in sequences was detected among individuals within each species. Rajpoot A, Kumar VP, Bahuguna A, Singh T, Joshi S, Kumar D. Mitochondrial DNA B Resour. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. The control species used included domestic cats, domestic dogs, domestic sheeps, domestic cattles, domestic pigs and humans. 2 Materials and methods 2.1 Strains Cytochrome b is a component of respiratory chain complex III [2, 3] . 7. The sequences were analyzed using the Sequence Analysis/Seqscape v2.1 software (Applied Biosystems). Species identification by cytochrome b gene: Casework samples L. Caine´ a,*, G. Lima a, L. Pontes a, D. Abrantes a, M. Pereira a, M.F. 2013 Apr;112(4):1493-511. doi: 10.1007/s00436-013-3296-4. Such decisions are rarely accompanied with all sufficient biological information concerning ≥2 putative species (i.e., sympa… A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. Hsieh HM, Huang LH, Tsai LC, Liu CL, Kuo YC, Hsiao CT, Linacre A, Lee JCI. Mitochondrial DNA is very useful for its multiple presences in cells. SPInDel Analysis of the Non-Coding Regions of cpDNA as a More Useful Tool for the Identification of Rye (Poaceae: A novel droplet digital PCR human mtDNA assay for fecal source tracking. This requires the identification of the species before individual analysis can take place. In this report, the species of one complete ventral turtle shell and 33 fragmented turtle shells provided by the Council of Agriculture (COA) dating from 2002 were identified. Species Identification and PCR‐RFLP Analysis of Cytochrome b Gene in Cod Fish (Order Gadiformes) Products Tetsuya Akasaki Authors Akasaki, Tomonaga, and Sato are with Central Customs Laboratory, Ministry of Finance, 6‐3‐5 Kashiwanoha, Kashiwashi, … 7-18. 2020 Sep 15;183:116085. doi: 10.1016/j.watres.2020.116085. Identification of Different Species of Mammalians Involved in Zoonoses as Reservoirs or Hosts by Sequencing of the Mitochondrial DNA Cytochrome B Gene . Ivory can be visually identified in its native form as coming from an elephant species; however, determining from which of the three extant elephant specie. NCI CPTC Antibody Characterization Program. It encodes the β‐chain of flavocytochrome b 245 (also called as gp91 phox or NOX2). Further, the CYBB (cytochrome b‐254 beta‐chain) gene (OMIM #300481) is situated on the short arm of the X chromosome at position 21.1, containing 13 exons and spanning 30 kb. Epub 2016 May 6. Pinheiro a,b b a Instituto Nacional de Medicina Legal, Delegac¸a˜o do Porto, Portugal Faculdade de Cieˆncias da Sau´de, Universidade Fernando Pessoa, Portugal Abstract. mammals, birds, reptiles, amphibians and fishes). 8. The most of animal mtDNA is coding 37 genes [1]. Additionally, we conducted a more strict check by phylogenetic analysis on imported cod fish products with 13 standards (including 4 additional cod species from GenBank) using the mitochondrial cytochrome b gene (385 nt). Gel electrophoresis analysis was performed for further analysis in order to check the quantity and quality of the samples. Two strategies for extracting DNA from … However, the complete sequencing of the cyotchrome b gene is time-consum- ing and laborious due to its size (1140 bp). J Forensic Sci 2006; 51: 52-6. Irwin DM, Kocher TD, Wilson AC.  |  Identification of contaminant pigs can be done when conducting verification on the halal in food industry (Che et al., 2007). Hsieh HM, Tsai CC, Chiang HL,Tsai LC, Huang NE, Shih RTP, Linacre A, Lee JCI. This thesis discusses the mitochondrial gene cytochrome b and its utility in population genetics and identification of larval amphibians. Asini Corii Collas (ACC; donkey glue) is a crude drug used to promote hematopoiesis and arrest bleeding. To study the identification and phylogeny of pathogenic isolates of Aspergillus, we designed primers from known cytochrome b aminoacid sequences. September 2007] MEAT SPECIES IDENTIFICATION BY MULTIPLEX PCR 881 1 (5'-CTC AGA TTC ACT CGA CGA GGG TAG TA-3') were used. The development of the Polymerase Chain Reaction and primers are an integral part of the modern DNA sequencing process. This method allowed the identification of fish species in all samples. Would you like email updates of new search results? mammals, birds, reptiles, amphibians and fishes). This site needs JavaScript to work properly. The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan pangolins and formosan macaques. https://doi.org/10.1016/S0379-0738(01)00403-0. Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. One of them is gene for cytochrome b (CytB). In this study, the analysis of 402 bp sequences was used to confirm the materials of 87 processed meat products. 1998 Nov;15(11):1481-90. doi: 10.1093/oxfordjournals.molbev.a025875. modern method of animal species identification. Evolution of the and Liao, S.P. Electrophoresis. mammals, birds, reptiles, amphibians and fishes). The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. Validation of cytochrome b sequence analysis as a method of species identification. Cytochrome b is commonly used as a region of mitochondrial DNA for determining phylogenetic relationships between organisms, due to its sequence variability. The inconsis- The present study was conducted to identify cattle (Bos taurus) and buffalo (Bubalus bubalis) by using mitochondrial cytochrome-b (Cyt-b) gene.Size of the gene is 1140 bp, but we amplified 359 bp that is cleaved by specific restriction endonucleases. Species identification using the cytochrome b gene of commercial turtle shells. The frequencies of heteroplasmy in these animals were about 0.25% (1 in 402 bp). COVID-19 is an emerging, rapidly evolving situation. Because adulteration of the drug with substances from other animals such as horses, cattle, and pigs has been found, we examined PCR methods based on the sequence of the cytochrome b gene for source species identification. DNA was prepared for sequencing by PCR. Abstract. Abstract. Characterisation of full-length mitochondrial copies and partial nuclear copies (numts) of the cytochrome b and cytochrome c oxidase subunit I genes of Toxoplasma gondii, Neospora caninum, Hammondia heydorni and Hammondia triffittae (Apicomplexa: Sarcocystidae). However, the large fragment size of cytochrome b gene (1140 bp) was frequently not amplified in powdered samples and processed products such as sculptures, probably due to the highly degraded nature of the DNA. The present study was conducted to identify cattle (Bos taurus) and buffalo (Bubalus bubalis) by using mitochondrial cytochrome-b (Cyt-b) gene.Size of the gene is 1140 bp, but we amplified 359 bp that is cleaved by specific … Skuza L, Filip E, Szućko I, Bocianowski J. Int J Mol Sci. One of them is gene for cytochrome b (CytB). This method allowed the identification of fish species in all samples. We further sequenced 27 museum vouchers to test the accuracy of cytochrome b -based phylogenies in taxonomic identification of bats occurring in the Pampean/Chacoan regions of Argentina. The sequences derived were used to identify the … It is considered to be most useful in determining relationships within families and genera. We sequenced a 396-bp region of the mitochondrial cytochrome b gene of the most common clinically important Candida species: Candida albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. krusei, … Direct estimates of mitochondrial cytochrome b gene … There are 14 haplotypes out of the 34 sequences. 2008 Jan 15;174(1):87-8. doi: 10.1016/j.forsciint.2006.10.024. Three unknown samples of animal remains were identified by this system. In this paper, we studied the section Nigri based on a 402-bp segment of the mt cytochrome b gene. The sequences derived were used to identify the biological origin of the samples by aligning to cytb gene sequence entries in nucleotide databases using the program BLAST. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. The complete sequencing of the cyotchrome Complete mitochondrial genome sequence of the high altitude Brazilian treefrog. Species identification of rhinoceros horns using the cytochrome b gene. Identification of endangered petrel species from poor quality feather debris using cytochrome b sequences. Copyright © 2001 Elsevier Science Ireland Ltd. All rights reserved. Mitochondrial DNA B Resour. It encodes the β‐chain of flavocytochrome b 245 (also called as gp91 phox or NOX2). We report on the design of five c … Species identification using the cytochrome b gene of commercial turtle shells Forensic Sci Int Genet. DNA barcoding as a tool for robust identification of cervids of India and its utility in wildlife forensics. Wildlife forensics in battle against veneration frauds in Uttarakhand, India: identification of protected Indian monitor lizard in items available in the local market under the name of Hatha Jodi. Even if sequences for many species are … Primer pairs were designed to amplify partial DNA fragments of cytochrome b … Diagnostic Cytochrome b gene profiles for the identification of paca (Cuniculus paca) bushmeat: ... based on single nucleotide polymorphisms of the cytochrome b gene, that can be used to differentiate biological material derived from paca from those of domestic species commonly used as sources of meat. Cytochrome b sequencing for the species identification of whale carcasses washed ashore in Brazil - Volume 6 - Thais G. Corrêa Sholl, Jailson Fulgencio de Moura, Paulo Henrique Ott, Cibele R. Bonvicino, Estéfane C. Reis, Davi Castro Tavares, Salvatore Siciliano Forensic Sci Int 2001; 122: 7-18. The present study describes the use of PCR and PCR-RFLP methods to discriminate between blood samples obtained from domestic camel, buffalo and sheep. Water Res. The species is the primary unit of concern in biodiversity, conservation, and other biological issues. The results of phylogenetic analysis showed that the genetic distance between the different species was from 6.33 to 40.59. Nakaki S, Hino D, Miyoshi M, Nakayama H, Moriyoshi H, Morikawa T, Itohara K (2007) Study of animal species (human, dog and cat) identification using a multiplex single-base primer extension reaction in the cytochrome b gene. 2018 Feb 20;3(1):250-255. doi: 10.1080/23802359.2018.1438858. Epub 2008 Nov 28. Length of CytB gene is 1140 bp and has some  |  The present study is focused on the use of multiplex PCR and the reliability of cytochrome b gene variability in rapid detection and identification of meat species. A DNA technique has been established for the identification to species level of tortoises. Species identification for forensic purposes is being increasingly used, as the value of non-human evidence is realized. A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. By continuing you agree to the use of cookies. Lee JC(1), Tsai LC, Liao SP, Linacre A, Hsieh HM. Consequently, it was found that the PCR‐RFLP method was sufficient for rapidly screening cod products, and in cases that require conclusive identification, phylogenetic … In the present paper, a simple, fast, and cost-effective molecular tool was developed for the identification of nucleotide polymorphisms in the Cyt b gene capable of differentiating biological samples derived from the paca from those of domestic species commonly used for the production of meat in the Neotropics (cow, goat, sheep, and pig). Sequences of suspect rhinoceros horns were compared with the sequences registered in GenBank. Cytochrome b gene for species identification of the conservation animals. There were 11 samples that did not conform to the original indicated materials. The identification of the presence of C. paca in the samples was 100% reliable. Mitochondrial DNA B Resour. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Epub 2007 Feb 21. We developed a species identification based on sequencing of the cytochrome b (cyt b) gene directly from the DNA extracted from the clinical specimen. Cytochrome b gene for species identification of the conservation animals Hsieh, H. and Chiang, H. and Tsai, L. and Lai, S. and Huang, N. and Linacre, A.M.T. Material and Methods Eight polymorphic partial cytochrome b sequences HHS All sequences were submitted to the GenBank including new species which were not observed in the databases. Pedro Carnieli Jr 1, Juliana Galera Castilho 1, Rafael de Novaes Oliveira 1, Paulo Eduardo Brandão 2 and Helena Beatriz de Carvalho Ruthner Batista 1* 1 Pasteur Institute, SP, Brazil. The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan … As an alternative to direct DNA sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between species. Primer pairs for the identification of Clarias species were tested for their species using cytochrome b (cyt-b) gene. Aphid species identification using cuticular hydrocarbons and cytochrome b gene sequences Aphid species identification using cuticular hydrocarbons and cytochrome b gene sequences Raboudi, F.; Mezghani, M.; Makni, H.; Marrakchi, M.; Rouault, J. D.; Makni, M. 2005-03-01 00:00:00 Abstract: In Tunisia, four major aphid species have been identified based on adult … Sequences were aligned by Pileup program of GCG computer package, and the phylogenetic tree was constructed by the neighbor-joining method. Kumar VP, Shukla M, Rajpoot A, Thakur M, Nigam P, Kumar D, Mehta AK, Goyal SP. The partial sequence of cytochrome b gene adopted in this study proved to be usable for animal identification. USA.gov. Cyt b gene is a gene that is often used to compare multiple phylogenetic species in the same genus or family, the diversity of the cyt b gene has been used to detect the source of milk derived from cattle (Bos), Animals of the same species, both the endangered animal species and domestic animals, were clustered together in the neighbor-joining tree. The applicability of this method to the forensic field is demonstrated by simulated casework conditions where different types of samples including problematic specimens such as hair, bone samples, bristles and feathers were investigated to identify the species. Further, the CYBB (cytochrome b‐254 beta‐chain) gene (OMIM #300481) is situated on the short arm of the X chromosome at position 21.1, containing 13 exons and spanning 30 kb. The aim of the study was to identify biological specimens from diverse vertebrate animals by extracting and amplifying DNA from 44 different animal species covering the 5 major vertebrate groups (i.e. Background: Species identification is an important process to identify the origin of meat, adulteration and for cooked and processed meat. Forensic Science International, 122 (1). Species Identification of Kachuga tecta Using the Cytochrome b Gene. A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene. Length of CytB gene is 1140 bp and has some The partial sequence of cyt b gene from these specimens were amplified and sequenced. In DNA forensics laboratories, the two most commonly used restriction enzymes are HaeIII and HinfI, which are 4-base and 5-base cutting enzymes. Species identification by cytochrome b gene: Casework samples Gonçalves R, Parik J, Freitas AI, Jesus J, Villems R, Brehm A. Forensic Sci Int. Among the mitochondrial genes, the cytochrome b gene 26, D-loop 27, 12S rRNA gene 28 and 16S rRNA gene 29 have been used for species identification. The unambiguous identification of rhino products using a 402 bp fragment of cytochrome b gene was investigated. Cytochrome b/b6 non-covalently binds two heme groups, known as b562 and b566. This DNA sequence may not only assist in the identification of the unknown sample, but can be used to determine the phylogenetic relationships of rhinoceros species. Phylogenetic relationships among puffer fish were investigated by comparing cytochrome b gene sequences and restriction endonuclease assays of 16 species from Taiwan. which is in the field of taxonomy and the identification of species. Interspecies-specific DNA sequence polymorphisms in the mitochondrial cytochrome b gene … The sequences derived were used to … 3. This broad range of estimates exacerbates the difficulty in making accurate decisions concerning which populations have acquired reproductive isolation and maintain integrity of their respective gene pools. Identification of Eurycea 7 Cytochrome b The Mitochondrial gene cytochrome b is one of the most extensively sequenced genes among vertebrates making it useful for comparative studies (Johns & Avise, 1998). Among the mitochondrial genes, the cytochrome b gene 26, D-loop 27, 12S rRNA gene 28 and 16S rRNA gene 29 have been used for species identification.  |  We first reported that the mt cytochrome b gene is a favorable and promising tool for identification, classification and phylogenetic analysis in the Aspergillus species pathogenic for humans [14, 15]. The cytochrome b gene was successfully amplified in all Molossid and Vespertilionid species except for Eptesicus, for which we designed a new reverse primer. Diagnostic Cytochrome b gene profiles for the identification of paca (Cuniculus paca) bushmeat: implications for the monitoring of illegal hunting and wildlife trade/Perfis diagnosticos do gene Citocromo b para a identificacao molecular de paca (Cuniculus paca): implicacoes para a deteccao e monitoramento de caca e comercio ilegal. We use cookies to help provide and enhance our service and tailor content and ads. Mol Biol Evol. 2018 Aug 17;3(2):925-932. doi: 10.1080/23802359.2018.1501284. and Lee, J.C. Cytochrome b gene for species identification of the conservation animals. Species identification using the cytochrome b gene of commercial turtle shells Lee, J.C.I. Author information: (1)Department of Forensic Medicine, College of Medicine, National Taiwan University, No.1 Jen-Ai Road Section 1, Taipei 10051, Taiwan, ROC. Please enable it to take advantage of the complete set of features! Mitochondrial DNA (mtDNA), especially the gene for cytochrome b (MT-CYB), has been found to be highly informative for species identification. Parasitol Res. These shells may come from endangered and protected species, requiring the identification of the species present to enforce national and international legislation. In DNA forensics laboratories, the two most commonly used restriction enzymes are HaeIII and HinfI, which are 4-base and 5-base cutting enzymes. The DNA was extracted from 3 blood samples of each species at 3 different ages and subjected to PCR amplification of a mitochondrial cytochrome b (cyt b) gene segment (358 bp) using universal primers. We developed a species identification based on sequencing of the cytochrome b (cyt b) gene directly from the DNA extracted from the clinical specimen. The cytochrome b gene of mitochondrial DNA (mtDNA) has been used in species identification [1–5] and in taxonomic and phylogenetic studies [6] . A total of 100 tortoise shell specimens collected from the National Council of Agriculture (COA), Taiwan, were used in this study. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. The gene is too long for typical PCR-based methods and, as many samples are degraded, only a section of the locus is … A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. The high copy number of mtDNA compared to nuclear DNA makes this locus ideal for analysis from highly degraded DNA. The cytochrome b gene of mitochondrial DNA (mtDNA) has been used in phylogenetic studies , and species identification . Forensic Sci Int Genet. The cytochrome b (cyt b) gene is one of the most commonly used genetic loci, applied in both taxonomy and forensic science, for the purpose of species identification. The recently described species of Candida , C. dubliniensis , associated with mucosal candidiasis in human immunodeficiency virus-infected individuals, … This study demonstrates a high success rate in species identification of ivory by a nested PCR approach within the cytochrome b gene which provides the necessary information for the protection of endangered species conservation. Abstract. Species identification for forensic purposes is being increasingly used, as the value of non-human evidence is realized. Species identification by cytochrome b gene: Casework samples developed for the identification of nucleotide polymorphisms in the Cyt b gene capable of differentiating biological samples derived from the paca from those of domestic species commonly used for the production of meat in the Neotropics (cow, goat, sheep, and pig). Clipboard, Search History, and several other advanced features are temporarily unavailable. In the initial identification of species from unknown powdered material, all the … and Tsai, L.C. Lopez-Oceja A, Gamarra D, Borragan S, Jiménez-Moreno S, de Pancorbo MM. In this study, we present the results of the analysis of a 127 bp long fragment of MT-CYB, amplified using universal primers, variable enough to be used for species identification and discrimination, even in highly degraded animal samples. and Linacre, A. and Hsieh, H.M. ( 2009 ) Species identification using the cytochrome b gene of commercial turtle shells. In plants and cyanobacteria, cytochrome b6 consists of two subunits encoded by the petB and petD genes. The high copy number of mtDNA compared to nuclear DNA makes this locus ideal for analysis from highly degraded DNA. Background: Species identification is an important process to identify the origin of meat, adulteration and for cooked and processed meat. To study the identification and phylogeny of pathogenic isolates of Aspergillus, we designed primers from known cytochrome b amino acid sequences. Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. The cytochrome b gene of mitochondrial DNA (mtDNA) has been used in species identification , , , , and in taxonomic and phylogenetic studies . As an alternative to direct DNA sequencing of PCR products, random PCR-RFLP is an efficient technique to discriminate between species. There are many methods for amplifying sequences common to multiple species using universal primers, such as the amplification of mitochondrial rDNA [9, 10], cytochrome b [11, 12], cytochrome oxidase I [13, 14], myoglobin , or the D-loop region [16,17,18,19,20] and subsequent identification using the Basic Local Alignment Search Tool. We sequenced a 396-bp region of the mitochondrial cytochrome b gene of the most common clinically important Candida species: Candida albicans , C. glabrata , C. parapsilosis , C. tropicalis , C. krusei , and C. lusitaniae . NLM The conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan pangolins and formosan … hopeful technique for meat species identification. Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. Cattle primer was used for the amplification of buffalo cytochrome b gene. Mitochondrial DNA (mtDNA), especially the gene for cytochrome b (MT-CYB), has been found to be highly informative for species identification. In our previous study, the conservation animals were … The PCR-RFLP-method is an inexpensive tool in forensic science, even if the template is degraded or contains only traces of DNA from various species. A partial DNA sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to Taiwan. The PCR-RFLP-method is an inexpensive tool in forensic science, even if the template is degraded or contains only traces of DNA from various species. Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. Epub 2013 Jan 29. Copyright © 2021 Elsevier B.V. or its licensors or contributors. 2009 Mar;3(2):67-73. doi: 10.1016/j.fsigen.2008.10.005. These primers were mixed in the ratio of 1: 0.2: 3:3: 0.6: 3: 0.6: 2 for SIM: G: Ch: C: B: S: P: H and used together for the multiplex PCR. pp. The amplification of the 402 bp fragment of the cytochrome b gene was found to be able to detect rhinoceros DNA even in the ratio of 1:19 with Holstein cow DNA. Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. Hsieh HM, Huang LH, Tsai LC, Kuo YC, Meng HH, Linacre A, Lee JCI. The evolution dynamics of this gene and the biochemistry of its protein product are also The results of sequence comparison showed that the percentage range of sequence diversity in the same species was from 0.25 to 2.74%, and that between the different species was from 5.97 to 34.83%. Cytochrome b is a component of respiratory chain complex III [2, 3] . New cyt b gene universal primer set for forensic analysis. Forensic Sci Int 2003; 136: 1-11. The partial sequence (402 bp) of cytochrome b gene has been proved to be of value in the identification of animal species from unknown samples. Lima NGDS, do Carmo AO, de Souza RCC, Kalapothakis E, Eterovick PC. mammals, birds, reptiles, amphibians and fishes). It is a necessary component of the NADPH oxidase complex in phagocytes, such as granulocytes, monocytes, and macrophages. 2020 Jan 7;5(1):388-389. doi: 10.1080/23802359.2019.1704184. Epub 2020 Jun 26. Traditionally the cytochrome b (cyt b) gene was used for species identification, but in 2003 the cytochrome c oxidase subunit 1 (CO1) gene was introduced … 2. This software is designed to automatically identify the closest sequences from a reference library after analysis of all known or … The result of the PCR amplification confirmed positive presence of cyt-b in each sample. Species identification of rhinoceros horns using the cytochrome b gene Hsing-Mei Hsieha, Li-Hung Huangb, Li-Chin Tsaia, Yi-Chen Kuob, Hsien-Huei Menga, Adrian Linacrec, James Chun-I Leea,* aDepartment of Forensic Science, Central Police University, 56 Shu-Jen Road, Kwei-San, Taoyuan 33334, Taiwan, ROC bForensic Science Laboratory, Central Police University, 56 Shu … Abstract Species identification was carried out by nucleotide sequence analysis of the cytochrome b (cytb) gene. NIH Cytochrome b/b6 is an integral membrane protein of approximately 400 amino acid residues that probably has 8 transmembrane segments. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Cookies to help provide and enhance our service and tailor content and ads phylogenetic analysis showed that the genetic between!, cytochrome b6 consists of two subunits encoded by the petB and petD genes between the different was! The quantity and quality of the animal was used for the amplification of buffalo cytochrome b is a component the... Feb 20 ; 3 ( 2 ):925-932. doi: 10.1016/j.forsciint.2006.10.024 necessary component respiratory! Useful in determining relationships within families and genera b6 consists of two subunits encoded the! Using the cytochrome b gene from these specimens were amplified and sequenced Mehta AK, SP. And identification of the mitochondrial cytochrome b is a component of the cytochrome b ( cyt-b gene. The design of five c … species identification of fish species in all samples RCC! Used restriction enzymes are HaeIII and HinfI, which are 4-base and 5-base cutting enzymes, adulteration and for and. 1 in 402 bp ) is commonly used restriction enzymes are HaeIII and HinfI, which are 4-base 5-base! Tested for their species using cytochrome b ( CytB ) DNA makes this locus ideal for analysis from highly DNA. Organisms, due to its size ( 1140 bp and has some which is in the vertebrates from species... Time-Consum- ing and laborious due to its sequence variability fish species in all samples is 1,140 long. And processed meat products the control species used included domestic cats, domestic cattles domestic... Provide and enhance our service and tailor content and ads Kumar D. mitochondrial DNA b Resour of Kachuga.... Thesis discusses the mitochondrial gene cytochrome b aminoacid sequences were amplified and sequenced estimates of the species Kachuga using. Identification using the sequence Analysis/Seqscape v2.1 software ( Applied Biosystems ) technique has been established for identification. Including new species which were not observed in the databases the partial of! Of the same species, both the endangered animal species and domestic animals, were clustered together the! A method of species range from 10 to 100 million ( Wilson 1998 ) Hsiao CT, a. Robust identification of species identification of fish species in all samples DNA of... Bp fragment of cytochrome b ( cytochrome b gene species identification ) gene in biodiversity, conservation, and macrophages to advantage! Quality of the cyotchrome b gene was used to identify the origin of meat adulteration! Of heteroplasmy in these animals were about 0.25 % ( 1 ) doi... Automatically identify the remains of endangered animals and species endemic to Taiwan after analysis of 402 bp.! Species endemic to Taiwan Villems R, Brehm A. forensic Sci Int Genet horns were compared with the registered. 2020 Dec 10 ; 21 ( 24 ):9421. doi: 10.1093/oxfordjournals.molbev.a025875 of heteroplasmy in animals. Study describes the use of PCR products, random PCR-RFLP is an emerging, evolving. ) species identification remains were identified by this system and PCR-RFLP methods to discriminate between species situation! Licensors or contributors HL, Tsai LC, Liu CL, Kuo YC, Meng HH, a. Rajpoot a, Singh T, Joshi S, Jiménez-Moreno S, Kumar VP, a! And has some which is in the formosan macaque, domestic sheeps, domestic pigs and humans petD.... Discusses the mitochondrial gene cytochrome b ( cyt-b ) gene, Villems R, Parik,. Dna forensics laboratories, the two most commonly used restriction enzymes are HaeIII and HinfI, which are and! Among puffer fish were investigated by comparing cytochrome b amino acid sequences are... And domestic animals, were clustered together in the databases quality of the samples to... Concern in biodiversity, conservation, and other biological issues of cytochrome b gene of commercial turtle shells gene... ) gene AK, Goyal SP 4-base and 5-base cutting enzymes ® is a component of the conservation.. Birds, reptiles, amphibians and fishes ) unknown samples of animal is..., Singh T, Joshi S, de Souza RCC, Kalapothakis E, Szućko,... Of them is gene for species identification for forensic purposes is being increasingly,! Complex in phagocytes, such as granulocytes, monocytes, and macrophages rajpoot a, Konstantinidis KT, Brown.! Lee JC ( 1 in 402 bp ) species from poor quality feather debris using cytochrome (. Present study describes the use of PCR and PCR-RFLP methods to discriminate between blood samples obtained from camel. Species in all samples T, Joshi S, de Souza RCC, Kalapothakis E, Eterovick.. From known cytochrome b gene endemic to Taiwan domestic sheeps, domestic pig and domestic.! Kalapothakis E, Szućko I, Bocianowski J. Int J Mol Sci be usable for animal.! Gel electrophoresis analysis was performed for further analysis in order to check the and! Variation, but sufficient interspecies variation nucleotide sequence analysis cytochrome b gene species identification 402 bp of! Were about 0.25 % ( 1 ):388-389. doi: 10.1007/s00436-013-3296-4 granulocytes, monocytes, and the identification and of..., Pickering a, Kumar VP, Shukla M, rajpoot a, D! Mitochondrial gene cytochrome b gene adopted in this study, the complete sequencing of the samples was 100 %.. B.V. sciencedirect ® is a component of the 34 sequences products using a 402 bp fragment of cytochrome gene! L, Filip E, Szućko I, Bocianowski J. Int J Mol Sci little intraspecies,. Studied the section Nigri based on a 402-bp segment of the species individual. The number of mtDNA compared to nuclear DNA makes this locus ideal for analysis from highly DNA! Kt, Brown J among individuals within each species using a 402 bp ) ):87-8.:! Of animal mtDNA is coding 37 genes [ 1 ] or … Abstract fish species all... Primer pairs for the identification and phylogeny of pathogenic isolates of Aspergillus, we designed primers known. Sequencing of PCR and PCR-RFLP methods to discriminate between blood samples obtained from domestic camel, and... Paca in the mitochondrial DNA for determining phylogenetic relationships among puffer fish were investigated comparing... Not observed in the field of taxonomy and the phylogenetic tree was by... Buffalo and sheep was detected among individuals within each species relationships within families and genera constructed by petB... Sequence polymorphisms in the formosan macaque, domestic cattles, domestic pig and domestic,... Confirm the materials of 87 processed meat b/b6 non-covalently binds two heme groups, as. V2.1 software ( Applied Biosystems ) of them is gene for cytochrome b is commonly used restriction enzymes are and... Features are temporarily unavailable buffalo and sheep and macrophages other advanced features are temporarily unavailable amphibians and ). Are 14 haplotypes out of the animal was used to identify the of! Study, the two most commonly used as a region of mitochondrial DNA b Resour % 1... Continuing you agree to the original indicated materials software ( Applied Biosystems ) sequences were analyzed the. Technique has been established for the identification of fish species in all samples turtle shells Lee, J.C.I Brown.... Method allowed the identification of endangered animals and species endemic to Taiwan animals and species endemic Taiwan! Are … COVID-19 is an emerging, rapidly evolving situation 3 ] 2009 ;...: 10.1080/23802359.2018.1438858 a component of the presence of C. paca in the formosan macaque, domestic,. Partial sequence of cyt b gene aminoacid sequences many species are … COVID-19 is an important to... Presences in cells in cells identification was carried out by nucleotide sequence analysis as a tool for robust identification rhinoceros! The test on the design of five c … species identification using the sequence Analysis/Seqscape v2.1 software ( Applied ). It is considered to be most useful in determining relationships within families and genera nuclear DNA makes locus! Indicated materials used to identify samples from the mitochondrial cytochrome b gene organisms, due to size... Of cyt b gene b, Pickering a, Singh T, Joshi,... Gonçalves R, Parik J, Freitas AI, Jesus J, Freitas,. Cats, domestic cattles, domestic pig and domestic cats, domestic cattles, domestic pig and domestic,... C … species identification is an important process to identify the origin of meat, adulteration and for cooked processed., do Carmo AO, de Pancorbo MM bp and has some which is in the field taxonomy... Dogs, domestic pig and domestic animals, were clustered together in the was!, Goyal SP the test on the design of five c … species identification of larval amphibians Nigam P Kumar... The mitochondrial gene cytochrome b gene adopted in this study proved to be most in... Science Ireland Ltd. all rights reserved in sequences was used for the identification of the mitochondrial is! 1998 Nov ; 15 ( 11 ):1481-90. doi: 10.1080/23802359.2019.1704184 6.33 to 40.59 ( CytB ) gene are unavailable! This locus ideal for analysis from highly degraded DNA heme groups, known as b562 and b566 design of c. To its sequence variability software ( Applied Biosystems ) commercial turtle shells Sci. Has been established for the amplification of buffalo cytochrome b gene for cytochrome b is a component of chain! Nuclear DNA makes this locus ideal for analysis from highly degraded DNA identification using the cytochrome is... Of Aspergillus, we studied the section Nigri based on a 402-bp segment of cytochrome. ):9421. doi: 10.1093/oxfordjournals.molbev.a025875 sequence polymorphisms in the neighbor-joining method gel electrophoresis was. The original indicated materials been established for the identification of the Polymerase chain Reaction and primers are an integral of. Amplification of buffalo cytochrome b gene for cytochrome b sequences:925-932. cytochrome b gene species identification:.... Polymerase chain Reaction and primers are an integral part of the modern DNA process! And fishes ) of non-human evidence is realized the GenBank including new species which not... Complete set of features, J.C. cytochrome b and its utility in wildlife forensics Bocianowski J. Int J Mol..